SEMA4A - Antibodies - The Human Protein Atlas

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SEMA4A

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA053726

Antibody HPA066006

Antibody HPA069136

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA053726

HPA066006

HPA069136

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA053726

Antibody HPA066006

Antibody HPA069136

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA053726

Antibody HPA066006

Antibody HPA069136

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA069136.

Validated

Antibody staining overlaps with antibody HPA066006.

Standard validation

Approved

Supported

Figure description

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & cell junctions.

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.

Antibody dilution

1:65

1:130

1:132

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA053726

Antibody HPA066006

Antibody HPA069136

Standard validation

Supported

Figure description

Immunohistochemical staining of human tonsil shows strong membranous positivity in a subset of non-germinal center cells.

Expression

RNA: detected in 34 tissues
Protein: detected in 9 cell types

Retrieval

HIER pH6

Antibody dilution

1:2500

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA053726

Antibody HPA066006

Antibody HPA069136

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

83.6, 79.7, 69.1

Antibody dilution

1:320

1:430

1:440

PROTEIN ARRAY

Antibody HPA053726

Antibody HPA066006

Antibody HPA069136

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2600

1:5200

1:5250

ANTIGEN INFORMATION

Antibody HPA053726

Antibody HPA066006

Antibody HPA069136

Antigen

Recombinant protein fragment

Length (aa)

144

Antigen sequence

PNLNSWKQDMERGNPEWACASGPMSRSLRPQSRPQIIKEVLAVPNSILEL
PCPHLSALASYYWSHGPAAVPEASSTVYNGSLLLIVQDGVGGLYQCWATE
NGFSYPVISYWVDSQDQTLALDPELAGIPREHVKVPLTRVSGGA

LPFNVIRHAVLLPADSPTAPHIYAVFTSQWQVGGTRSSAVCAFSLLDIER
VFKGKYKELNKETSRWTTYRGPETNPRPGSCSVGPSSD

SGVEYTRLAVETAQGLDGHSHLVMYLGTTTGSLHKAVVSGDSSAHLVEEI
QLFPDPEPVRNLQLAPTQGAVFVGFSGGVWRVPRANCSVYE

Matching transcripts

SEMA4A-001 - ENSP00000357268 [100%]
SEMA4A-002 - ENSP00000347117 [100%]
SEMA4A-003 - ENSP00000357265 [100%]
SEMA4A-004 - ENSP00000357267 [100%]
SEMA4A-201 - ENSP00000357269 [100%]

Other gene match

ANTIGEN VIEW

SEMA4A-001	SEMA4A-002	SEMA4A-003	SEMA4A-004	SEMA4A-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.