Fields »
ABOUT
HELP
BLOG
ZKSCAN7

ANTIBODY INFORMATION
 
 

Antibody HPA048312

  

Antibody HPA049906

  

Antibody HPA071850

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA048312   HPA049906   HPA071850  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 ZNF852 - ENSG00000178917 [100%]
     ZNF852 - ENSG00000178917 [100%]
  

Released in version

 10   10   16  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA048312

  

Antibody HPA049906

  

Antibody HPA071850

  
Immunocytochemistry

  Approved   Approved  
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Supported   Supported   Supported  
Protein array

Approved   Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048312

  

Antibody HPA049906

  

Antibody HPA071850

  

Standard validation

   Approved   Approved  

     

Figure description

   Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.   Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.  

Antibody dilution

   1:82   1:48  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048312

  

Antibody HPA049906

  

Antibody HPA071850

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunohistochemical staining of human kidney shows cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human kidney shows cytoplsmic positivity in cells in tubules.    

Expression

 RNA: detected in 23 tissues
Protein: detected in 53 cell types		   RNA: detected in 23 tissues
Protein: detected in 48 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:350   1:150    

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA048312

  

Antibody HPA049906

  

Antibody HPA071850

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 85, 62.1, 60.5, 41.5   85, 41.5, 30.8   85, 62.1, 60.5, 41.5  

Antibody dilution

 1:320   1:410   1:160  

PROTEIN ARRAY
 
 

Antibody HPA048312

  

Antibody HPA049906

  

Antibody HPA071850

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2550   1:3250   1:1950  

ANTIGEN INFORMATION
 
 

Antibody HPA048312

  

Antibody HPA049906

  

Antibody HPA071850

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 31   74   73  

Antigen sequence

 
YKEVEEHPPLSSSPVEHEGVLKGQKSYRCDE
  
PAQKQEMHFEETTALGTTKESPPTSPLSGGSAPGAHLEPPYDPGTHHLPS
GDFAQCTSPVPTLPQVGNSGDQAG
  
MMESSELTPKQEIFKGSESSNSTSGGLFGVVPGGTETGDVCEDTFKELEG
QPSNEEGSRLESDFLEIIDEDKK
 

Matching transcripts

 ZKSCAN7-001 - ENSP00000273320 [97%]
ZKSCAN7-004 - ENSP00000395524 [97%]
ZKSCAN7-006 - ENSP00000405034 [97%]
  ZKSCAN7-001 - ENSP00000273320 [100%]
ZKSCAN7-002 - ENSP00000345404 [100%]
ZKSCAN7-003 - ENSP00000416681 [100%]
ZKSCAN7-004 - ENSP00000395524 [100%]
ZKSCAN7-006 - ENSP00000405034 [92%]
  ZKSCAN7-001 - ENSP00000273320 [86%]
ZKSCAN7-004 - ENSP00000395524 [86%]
ZKSCAN7-006 - ENSP00000405034 [86%]
 

Other gene match

 ZNF852 - ENSG00000178917 [100%]
     ZNF852 - ENSG00000178917 [100%]
  
ANTIGEN VIEW
ZKSCAN7-001
ZKSCAN7-002
ZKSCAN7-003
ZKSCAN7-004
ZKSCAN7-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.