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Antibody staining overlaps with antibody HPA066805.
Validated
Antibody staining overlaps with antibody HPA053925.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & cytosol.
Antibody dilution
1:73
1:114
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA053925
Antibody HPA060755
Antibody HPA066805
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human lateral ventricle shows moderate cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 42 cell types
Retrieval
HIER pH6
Antibody dilution
1:650
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA053925
Antibody HPA060755
Antibody HPA066805
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
64, 61.7, 30.1, 17.5
64, 61.7, 30.1, 17.5
64, 61.7, 30.1
Antibody dilution
1:360
1:150
1:380
PROTEIN ARRAY
Antibody HPA053925
Antibody HPA060755
Antibody HPA066805
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
