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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Antibody dilution
1:25
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA001928
Standard validation
Approved
Figure description
Immunohistochemical staining of human cerebellum shows moderate nuclear positivity in Purkinje cells, cells in molecular layer and cells in granular layer.
Expression
RNA: detected in 37 tissues Protein: detected in 66 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001928
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Figure description
Target mass (kDa)
273.7, 272.8, 268, 222.7, 221, 160.4
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA001928
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
