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ZNF239

ANTIBODY INFORMATION
 
 

Antibody HPA030843

  

Antibody HPA046679

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA030843   HPA046679  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   9  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA030843

  

Antibody HPA046679

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Uncertain  
Western blot

Uncertain   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030843

  

Antibody HPA046679

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies.    

Antibody dilution

 1:96    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030843

  

Antibody HPA046679

  

Standard validation

   Uncertain  

   

Figure description

   Immunohistochemical staining of human nasopharynx shows strong positivity in cilia of respiratory epithelial cells.  

Expression

   RNA: detected in 29 tissues
Protein: detected in 11 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:75  

Literature conformity

   No avaliable gene/protein characterization data.  

RNA consistency

   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030843

  

Antibody HPA046679

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 51.6   51.6  

Antibody dilution

 1:250   1:340  

PROTEIN ARRAY
 
 

Antibody HPA030843

  

Antibody HPA046679

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2750  

ANTIGEN INFORMATION
 
 

Antibody HPA030843

  

Antibody HPA046679

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 72   72  

Antigen sequence

 
IDTQDSSVKFCKNEPQDHQESRRLFVMEESTERKVIKGESCSENLQVKLV
SDGQELASPLLNGEATCQNGQL
  
IDTQDSSVKFCKNEPQDHQESRRLFVMEESTERKVIKGESCSENLQVKLV
SDGQELASPLLNGEATCQNGQL
 

Matching transcripts

 ZNF239-001 - ENSP00000307774 [100%]
ZNF239-002 - ENSP00000363569 [100%]
ZNF239-201 - ENSP00000398202 [100%]
ZNF239-202 - ENSP00000443907 [100%]
  ZNF239-001 - ENSP00000307774 [100%]
ZNF239-002 - ENSP00000363569 [100%]
ZNF239-201 - ENSP00000398202 [100%]
ZNF239-202 - ENSP00000443907 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZNF239-001
ZNF239-002
ZNF239-201
ZNF239-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.