Fields »
ABOUT
HELP
BLOG
ANXA4

ANTIBODY INFORMATION
 
 

Antibody HPA007393

  

Antibody CAB005076

  

Antibody CAB017560

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Santa Cruz Biotechnology
  

Product name

 HPA007393   sc-1930   sc-28827  

Host species

 Rabbit   Goat   Rabbit  

Clonality

 pAb   msAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Affinity  

Other gene match

      

Released in version

 3   3   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA007393

  

Antibody CAB005076

  

Antibody CAB017560

  
Immunocytochemistry

Approved      
Immunohistochemistry


Validated
   Approved  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA007393

  

Antibody CAB005076

  

Antibody CAB017560

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.      

Antibody dilution

 1:20      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007393

  

Antibody CAB005076

  

Antibody CAB017560

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

 

    
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human pancreas shows distinct positivity in ductal cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in intra-lobular ducts of exocrine pancreas.   Immunohistochemical staining of human pancreas shows strong positivity in intercalated ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 49 cell types		   RNA: detected in 37 tissues
Protein: detected in 42 cell types		   RNA: detected in 37 tissues
Protein: detected in 48 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:275   1:100   1:800  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007393

  

Antibody CAB005076

  

Antibody CAB017560

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 36.1, 33.6   36.1, 33.6   36.1, 33.6  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA007393

  

Antibody CAB005076

  

Antibody CAB017560

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA007393

  

Antibody CAB005076

  

Antibody CAB017560

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Recombinant protein  

Length (aa)

 128      

Antigen sequence

 
KGGTVKAASGFNAMEDAQTLRKAMKGLGTDEDAIISVLAYRNTAQRQEIR
TAYKSTIGRDLIDDLKSELSGNFEQVIVGMMTPTVLYDVQELRRAMKGAG
TDEGCLIEILASRTPEEIRRISQTYQQQ
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					ANXA4-001 - ENSP00000377833 [100%] 
ANXA4-002 - ENSP00000386756 [83%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ANXA4-001
ANXA4-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.