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Immunofluorescent staining of human cell line SH-SY5Y shows localization to nuclear bodies, plasma membrane & cytosol.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA067336
Standard validation
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 9 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
Antibody dilution
1:5000
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA067336
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
18.2
Antibody dilution
1:660
PROTEIN ARRAY
Antibody HPA067336
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
