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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & vesicles.
Antibody dilution
1:101
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA044156
Antibody HPA062151
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows strong positivity in spermatozoa and late spermatids.
Expression
RNA: detected in 1 tissues Protein: detected in 34 cell types
Retrieval
HIER pH6
Antibody dilution
1:175
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA044156
Antibody HPA062151
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408141)
Target mass (kDa)
87.9, 76.7, 71
87.9, 76.7, 71
Antibody dilution
1:250
1:340
PROTEIN ARRAY
Antibody HPA044156
Antibody HPA062151
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
