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ANTIBODY INFORMATION
 
 

Antibody HPA004426

  

Antibody CAB004647

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA004426   H00005660-M01  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 3   2  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA004426

  

Antibody CAB004647

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004426

  

Antibody CAB004647

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.    

Antibody dilution

 1:56    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004426

  

Antibody CAB004647

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity with a granular pattern in cells of tubules.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity(granular pattern) in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 71 cell types		   RNA: detected in 37 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004426

  

Antibody CAB004647

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 58.4, 58.1, 18.7   58.4, 58.1, 26.3, 18.7  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004426

  

Antibody CAB004647

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA004426

  

Antibody CAB004647

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 143    

Antigen sequence

 
TNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKE
ICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDV
YCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEE
   

Matching transcripts

 PSAP-001 - ENSP00000378394 [100%]
PSAP-005 - ENSP00000488331 [98%]
PSAP-201 - ENSP00000378392 [98%]
   

Other gene match

    
ANTIGEN VIEW
PSAP-001
PSAP-005
PSAP-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.