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Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & centrosome.
Antibody dilution
1:92
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA054961
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
Antibody dilution
1:1000
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054961
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
44.7, 17
Antibody dilution
1:460
PROTEIN ARRAY
Antibody HPA054961
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
