SIRPA - Antibodies - The Human Protein Atlas

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SIRPA

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA054437

Antibody HPA058511

Antibody CAB002776

Antibody CAB015122

Provider

Atlas Antibodies
Sigma-Aldrich

Chemicon

Santa Cruz Biotechnology

Product name

HPA054437

HPA058511

AB16949

sc-17803

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA054437

Antibody HPA058511

Antibody CAB002776

Antibody CAB015122

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA054437

Antibody HPA058511

Antibody CAB002776

Antibody CAB015122

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA058511 across 75 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA054437 across 75 cell types.

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.

Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer, neuropil was distinctly stained.

Immunohistochemical staining of human lateral ventricle shows moderate cytoplasmic positivity in neuronal cells

Immunohistochemical staining of human lateral ventricle shows strong positivity in neuropil.

Expression

RNA: detected in 37 tissues
Protein: detected in 6 cell types

RNA: detected in 37 tissues
Protein: detected in 8 cell types

RNA: detected in 37 tissues
Protein: detected in 42 cell types

RNA: detected in 37 tissues
Protein: detected in 47 cell types

Retrieval

HIER pH6

Antibody dilution

1:50

1:60

1:500

1:600

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA054437

Antibody HPA058511

Antibody CAB002776

Antibody CAB015122

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

55.4, 55

Antibody dilution

1:80

1:500

PROTEIN ARRAY

Antibody HPA054437

Antibody HPA058511

Antibody CAB002776

Antibody CAB015122

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA054437

Antibody HPA058511

Antibody CAB002776

Antibody CAB015122

Antigen

Recombinant protein fragment

Not known

Recombinant protein

Length (aa)

Antigen sequence

NNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYA
SVQVPRK

RIRQKKAQGSTSSTRLHEPEKNAREITQDTNDITYADLNLPKGKKPAPQA
AEPN

Matching transcripts

SIRPA-001 - ENSP00000351621 [100%]
SIRPA-002 - ENSP00000348307 [100%]
SIRPA-003 - ENSP00000478763 [100%]
SIRPA-201 - ENSP00000382941 [100%]

SIRPA-001 - ENSP00000351621 [100%]
SIRPA-002 - ENSP00000348307 [100%]
SIRPA-201 - ENSP00000382941 [100%]
SIRPA-003 - ENSP00000478763 [93%]

Other gene match

ANTIGEN VIEW

SIRPA-001	SIRPA-002	SIRPA-003	SIRPA-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.