CD2AP - Antibodies - The Human Protein Atlas

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CD2AP

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA003267

Antibody HPA003326

Antibody CAB004352

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA003267

HPA003326

sc-25272

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA003267

Antibody HPA003326

Antibody CAB004352

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA003267

Antibody HPA003326

Antibody CAB004352

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.

Antibody dilution

1:7

1:95

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA003267

Antibody HPA003326

Antibody CAB004352

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA003326 across 45 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA003267 across 45 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA003326 across 67 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA003267 across 67 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 50 cell types

RNA: detected in 37 tissues
Protein: detected in 57 cell types

RNA: detected in 37 tissues
Protein: detected in 55 cell types

Retrieval

HIER pH6

Antibody dilution

1:50

1:300

1:200

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA003267

Antibody HPA003326

Antibody CAB004352

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

71.5

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA003267

Antibody HPA003326

Antibody CAB004352

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2000

1:3000

ANTIGEN INFORMATION

Antibody HPA003267

Antibody HPA003326

Antibody CAB004352

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

127

150

Antigen sequence

WSGTLNNKLGLFPSNFVKELEVTDDGETHEAQDDSETVLAGPTSPIPSLG
NVSETASGSVTQPKKIRGIGFGDIFKEGSVKLRTRTSSSETEEKKPEKPL
ILQSLGPKTQSVEITKTDTEGKIKAKE

VDFDSLTVRTSKETDVVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGG
HSPTHSPEKILKLPKEEDSANLKPSELKKDTCYSPKPSVYLSTPSSASKA
NTTAFLTPLEIKAKVETDDVKKNSLDELRAQIIELLCIVEALKKDHGKEL

Matching transcripts

CD2AP-001 - ENSP00000352264 [100%]

Other gene match

ANTIGEN VIEW

CD2AP-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.