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QRICH1

ANTIBODY INFORMATION
 
 

Antibody HPA037677

  

Antibody HPA037678

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037677   HPA037678  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA037677

  

Antibody HPA037678

  
Immunocytochemistry


Validated

Validated
  

Validated
  
Immunohistochemistry

Approved   Approved  
Western blot


Validated
   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037677

  

Antibody HPA037678

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		    

 

Relative fluorescence intensity (RFI)

    

Figure description

 Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:39    
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA037678.		  
Validated

Antibody staining overlaps with antibody HPA037677.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:39   1:33  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037677

  

Antibody HPA037678

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts and Leydig cells.   Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts and Leydig cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:300  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037677

  

Antibody HPA037678

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 86.4    

Loading control

 Total protein image    

Antibody dilution

 1:87    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 86.4   86.4, 11.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA037677

  

Antibody HPA037678

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA037677

  

Antibody HPA037678

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 89   96  

Antigen sequence

 
EEHIPHQQIQAQLVAGQSLAGGQQIQIQTVGALSPPPSQQGSPREGERRV
GTASVLQPVKKRKVDMPITVSYAISGQPVATVLAIPQGQ
  
MNNSLENTISFEEYIRVKARSVPQHRMKEFLDSLASKGPEALQEFQQTAT
TTMVYQQGGNCIYTDSTEVAGSLLELACPVTTSVQPQTQQEQQIQV
 

Matching transcripts

 QRICH1-001 - ENSP00000378830 [100%]
QRICH1-003 - ENSP00000350094 [100%]
QRICH1-004 - ENSP00000412890 [100%]
  QRICH1-001 - ENSP00000378830 [100%]
QRICH1-003 - ENSP00000350094 [100%]
QRICH1-004 - ENSP00000412890 [100%]
QRICH1-012 - ENSP00000416133 [100%]
QRICH1-013 - ENSP00000413051 [100%]
QRICH1-014 - ENSP00000412870 [100%]
 

Other gene match

    
ANTIGEN VIEW
QRICH1-001
QRICH1-003
QRICH1-004
QRICH1-012
QRICH1-013
QRICH1-014
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.