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Immunofluorescent staining of human cell line RH-30 shows localization to nucleoli & cytosol.
Antibody dilution
1:122
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA023805
Antibody HPA023957
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human testis shows nuclear positivity in Leydig cells.
Immunohistochemical staining of human testis shows cytoplasmic positivity in Leydig cells.
Expression
RNA: detected in 33 tissues Protein: detected in 30 cell types
RNA: detected in 33 tissues Protein: detected in 23 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:75
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023805
Antibody HPA023957
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
66, 15.9, 15
66
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA023805
Antibody HPA023957
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
