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ZNF789

ANTIBODY INFORMATION
 
 

Antibody HPA029500

  

Antibody HPA050793

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA029500   HPA050793  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA029500

  

Antibody HPA050793

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA029500

  

Antibody HPA050793

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA050793.		  
Validated

Antibody staining overlaps with antibody HPA029500.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.  

Antibody dilution

 1:10   1:139  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA029500

  

Antibody HPA050793

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human small intestine shows strong luminal membranous positivity in glandular cells.    

Expression

 RNA: detected in 36 tissues
Protein: detected in 39 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:40    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA029500

  

Antibody HPA050793

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 50   50  

Antibody dilution

 1:250   1:700  

PROTEIN ARRAY
 
 

Antibody HPA029500

  

Antibody HPA050793

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:5600  

ANTIGEN INFORMATION
 
 

Antibody HPA029500

  

Antibody HPA050793

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 86   87  

Antigen sequence

 
CRLTFPTSGDEYSRGFLQNLNLIQDQNAQTRWKQGRYDEDGKPFNQRSLL
LGHERILTRAKSYECSECGKVIRRKAWFDQHQRIHF
  
FQFPKPEMICQLENWDEQWILDLPRTGNRKASGSACPGSEARHKMKKLTP
KQKFSEDLESYKISVVMQESAEKLSEKLHKCKEFVDS
 

Matching transcripts

 ZNF789-004 - ENSP00000331927 [100%]
  ZNF789-004 - ENSP00000331927 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZNF789-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.