PNP - Antibodies - The Human Protein Atlas

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PNP

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA001625

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA001625

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA001625

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA001625

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 32 cell types

Retrieval

HIER pH6

Antibody dilution

1:750

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA001625

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

32.1, 24.5

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA001625

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA001625

Antigen

Recombinant protein fragment

Length (aa)

155

Antigen sequence

YTYEDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYGEIP
NFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFH
LLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDER
GAPHR

Matching transcripts

PNP-001 - ENSP00000354532 [97%]
PNP-010 - ENSP00000452421 [97%]

Other gene match

ANTIGEN VIEW