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HMGN2

ANTIBODY INFORMATION
 
 

Antibody HPA077364

  

Antibody CAB018371

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   National Institutes of Health
  

Product name

 HPA077364   n/a  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

 HMGN4 - ENSG00000182952 [100%]
    

Released in version

 16   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA077364

  

Antibody CAB018371

  
Immunocytochemistry

Approved   Supported  
Immunohistochemistry

  Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA077364

  

Antibody CAB018371

  

Standard validation

 Approved   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.   Immunofluorescent staining of human cell line A-431 shows localization to nucleus.  

Antibody dilution

 1:28   1:200  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA077364

  

Antibody CAB018371

  

Standard validation

   Supported  

   

Figure description

   Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:1000  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA077364

  

Antibody CAB018371

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 9.5, 9.4   9.4  

Antibody dilution

 1:90   1:500  

PROTEIN ARRAY
 
 

Antibody HPA077364

  

Antibody CAB018371

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1000    

ANTIGEN INFORMATION
 
 

Antibody HPA077364

  

Antibody CAB018371

  

Antigen

 Recombinant protein fragment   Native protein  

Length (aa)

 21    

Antigen sequence

 
PKRKAKGDAKGDKAKVKDEPQ
   

Matching transcripts

 HMGN2-001 - ENSP00000355228 [95%]
HMGN2-201 - ENSP00000481160 [95%]
   

Other gene match

 HMGN4 - ENSG00000182952 [100%]
    
ANTIGEN VIEW
HMGN2-001
HMGN2-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.