OPA1 - Antibodies - The Human Protein Atlas

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OPA1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA036926

Antibody HPA036927

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA036926

HPA036927

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA036926

Antibody HPA036927

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Uncertain

Protein array

Approved

IMMUNOCYTOCHEMISTRY

Antibody HPA036926

Antibody HPA036927

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.

Antibody dilution

1:108

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA036926

Antibody HPA036927

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036927 across 73 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036926 across 73 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 66 cell types

RNA: detected in 37 tissues
Protein: detected in 62 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:150

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036926

Antibody HPA036927

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

117.7, 115.9, 113.7, 113.5, 111.8, 111.6

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA036926

Antibody HPA036927

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:12000

ANTIGEN INFORMATION

Antibody HPA036926

Antibody HPA036927

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

ESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEEALQARL
KDTENAIENMVGPDWKKRWLYWKNRTQEQCVHNETKNELEKMLKCNE

KEGCTVSPETISLNVKGPGLQRMVLVDLPGVINTVTSGMAPDTKETIFSI
SKAYMQNPNAIILCIQDGSVDAERSIVTDLVSQMDPHG

Matching transcripts

OPA1-001 - ENSP00000354681 [100%]
OPA1-002 - ENSP00000376233 [100%]
OPA1-201 - ENSP00000354781 [100%]
OPA1-202 - ENSP00000355324 [100%]
OPA1-203 - ENSP00000355311 [100%]
OPA1-204 - ENSP00000354429 [100%]

Other gene match

ANTIGEN VIEW

OPA1-001	OPA1-002	OPA1-201	OPA1-202	OPA1-203	OPA1-204

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.