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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Antibody dilution
1:100
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA027307
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human lymph node shows strong nuclear staining in reaction center cells while subsets of cells outside the reaction center displayed strong positivity the cytoplasm.
Expression
RNA: detected in 37 tissues Protein: detected in 43 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027307
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Target mass (kDa)
52.8, 33.9
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA027307
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
