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C1orf174

ANTIBODY INFORMATION
 
 

Antibody HPA008270

  

Antibody HPA065778

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA008270   HPA065778  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   16  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA008270

  

Antibody HPA065778

  
Immunocytochemistry


Validated

Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008270

  

Antibody HPA065778

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		    

 

Relative fluorescence intensity (RFI)

    

Figure description

 Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:30    
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA065778.		  
Validated

Antibody staining overlaps with antibody HPA008270.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.   Immunofluorescent staining of human cell line HaCaT shows localization to nucleus.  

Antibody dilution

 1:30   1:85  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008270

  

Antibody HPA065778

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human stomach shows moderate nuclear positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 47 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:50    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA008270

  

Antibody HPA065778

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 26   26  

Antibody dilution

 1:250   1:290  

PROTEIN ARRAY
 
 

Antibody HPA008270

  

Antibody HPA065778

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3450  

ANTIGEN INFORMATION
 
 

Antibody HPA008270

  

Antibody HPA065778

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 114   110  

Antigen sequence

 
VSDSRLAKTRDGLSVPKHSAGSGAEESNSSSTVQKQNEPGLQTEDVQKPP
LQMDNSVFLDDDSNQPMPVSRFFGNVELMQDLPPASSSCPSMSRREFRKM
HFRAKDDDDDDDDD
  
RLKARSCSAARLASAQEVAGSTSAKTACLTSSSHKATDRRTSKKFKCDKG
HLVKSELQKLVPKNDSASLPKVTPETPCENEFAEGSALLPGSEAGVSVQQ
GAASLPLGGC
 

Matching transcripts

 C1orf174-001 - ENSP00000355306 [100%]
  C1orf174-001 - ENSP00000355306 [99%]
 

Other gene match

    
ANTIGEN VIEW
C1orf174-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA008270

  

Antibody HPA065778

  

1.

 Mapping the subcellular protein distribution in three human cell lines
Fagerberg L et al
J Proteome Res 2011;10(8):3766-77
    

2.

 Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C et al
J Proteomics 2012;75(7):2236-51
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.