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CR1

ANTIBODY INFORMATION
 
 

Antibody HPA042455

  

Antibody HPA043579

  

Antibody HPA049348

  

Antibody CAB002491

  

Antibody CAB016271

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
   Santa Cruz Biotechnology
  

Product name

 HPA042455   HPA043579   HPA049348   NCL-CD35   sc-7308  

Host species

 Rabbit   Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

     CR1L - ENSG00000197721 [100%]
      

Released in version

 10   10   10   1   4  

References

          

VALIDATION SUMMARY
 
 

Antibody HPA042455

  

Antibody HPA043579

  

Antibody HPA049348

  

Antibody CAB002491

  

Antibody CAB016271

  
Immunohistochemistry

Supported   Supported   Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Supported   Uncertain   Uncertain  
Protein array

Approved   Approved   Approved      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042455

  

Antibody HPA043579

  

Antibody HPA049348

  

Antibody CAB002491

  

Antibody CAB016271

  

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells.   Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in white pulp.   Immunohistochemical staining of human tonsil shows distinct reticular positivity in germinal center.   Immunohistochemical staining of human tonsil shows cytoplasmic positivity in reaction center cells   Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells.  

Expression

 RNA: detected in 22 tissues
Protein: detected in 24 cell types		   RNA: detected in 22 tissues
Protein: detected in 39 cell types		   RNA: detected in 22 tissues
Protein: detected in 13 cell types		   RNA: detected in 22 tissues
Protein: detected in 12 cell types		   RNA: detected in 22 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:450   1:500   1:450   1:80   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042455

  

Antibody HPA043579

  

Antibody HPA049348

  

Antibody CAB002491

  

Antibody CAB016271

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

         

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 272.9, 223.7, 223.6   272.9, 223.7, 223.6, 72.9   272.9, 223.7, 223.6, 72.9, 62.7   272.9, 223.7, 223.6, 72.9   272.9, 223.7, 223.6, 72.9  

Antibody dilution

 1:250   1:250   1:260   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA042455

  

Antibody HPA043579

  

Antibody HPA049348

  

Antibody CAB002491

  

Antibody CAB016271

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:6000   1:2100      

ANTIGEN INFORMATION
 
 

Antibody HPA042455

  

Antibody HPA043579

  

Antibody HPA049348

  

Antibody CAB002491

  

Antibody CAB016271

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Not known   Not known  

Length (aa)

 67   34   57      

Antigen sequence

 
LIGSPSTTCLVSGNNVTWDKKAPICEIISCEPPPTISNGDFYSNNRTSFH
NGTVVTYQCHTGPDGEQ
  
GNNAHENPKEVAIHLHSQGGSSVHPRTLQTNEEN
  
VKCQALNKWEPELPSCSRVCQPPPDVLHAERTQRDKDNFSPGQEVFYSCE
PGYDLRG
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CR1-001 - ENSP00000383744 [100%] 
CR1-002 - ENSP00000356018 [100%] 
CR1-003 - ENSP00000356020 [100%] 
CR1-007 - ENSP00000356019 [100%] 
CR1-012 - ENSP00000356016 [100%] 

											 
																						CR1-001 - ENSP00000383744 [100%] 
CR1-002 - ENSP00000356018 [100%] 
CR1-003 - ENSP00000356020 [100%] 
CR1-007 - ENSP00000356019 [100%] 
CR1-011 - ENSP00000434718 [100%] 
CR1-012 - ENSP00000356016 [100%] 

											 
																						CR1-001 - ENSP00000383744 [100%] 
CR1-002 - ENSP00000356018 [100%] 
CR1-003 - ENSP00000356020 [100%] 
CR1-007 - ENSP00000356019 [100%] 
CR1-011 - ENSP00000434718 [100%] 
CR1-012 - ENSP00000356016 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						CR1L - ENSG00000197721 [100%]

											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CR1-001
CR1-002
CR1-003
CR1-007
CR1-011
CR1-012
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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