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ZDBF2

ANTIBODY INFORMATION
 
 

Antibody HPA039121

  

Antibody HPA050311

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039121   HPA050311  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA039121

  

Antibody HPA050311

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Uncertain  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039121

  

Antibody HPA050311

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:200   1:156  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039121

  

Antibody HPA050311

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human prostate shows strong cytoplasmic, membranous and nuclear positivity in glandular cells.    

Expression

 RNA: detected in 35 tissues
Protein: detected in 64 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:2000    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA039121

  

Antibody HPA050311

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

    

Target mass (kDa)

 265.6, 265.1   265.6, 265.1  

Antibody dilution

 1:250   1:780  

PROTEIN ARRAY
 
 

Antibody HPA039121

  

Antibody HPA050311

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:60000   1:60000  

ANTIGEN INFORMATION
 
 

Antibody HPA039121

  

Antibody HPA050311

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 88   82  

Antigen sequence

 
TDMPSNKGIFEDTIAKNHEEFFSNMDCTQEEKHLVFNKTAFWEQKCSVSS
EMKFDCISLQSASDQPQETAQDLSLWKEEQIDQEDNYE
  
KISSDCDDILHLVTNQSQMIVKEISLQNARHISLVDQSYESSSSETNFDC
DASPQSTSDYPQQSVTEVNLPKEVHIGLVDKN
 

Matching transcripts

 ZDBF2-001 - ENSP00000363545 [100%]
ZDBF2-201 - ENSP00000478626 [100%]
  ZDBF2-001 - ENSP00000363545 [100%]
ZDBF2-201 - ENSP00000478626 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZDBF2-001
ZDBF2-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.