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POU5F1

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GENERAL INFORMATION

Gene name

 POU5F1

Gene description

 POU class 5 homeobox 1

Protein class

 Cancer-related genes
Predicted intracellular proteins
Transcription factors

Predicted localization

 Intracellular

Number of transcripts

 8
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Mainly localized to the nucleoplasm. In addition localized to the cytosol.

RNA cell category

 Cell line enhanced (EFO-21)

Protein evidence

 Evidence at protein level

Main location

 Localized to the Nucleoplasm (supported)

Additional location

 In addition localized to the Cytosol (supported)

Single-cell variation

 Single-cell variation in protein expression observed.

DATA RELIABILITY

Reliability score

 Supported based on 2 antibodies.
CAB026380 , HPA058267
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ASSAYS
RNA expression
Human cells

RNA EXPRESSION OVERVIEW
RNA cell line category: Cell line enhanced (EFO-21)
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Mainly localized to the nucleoplasm. In addition localized to the cytosol.

Main location

 Nucleoplasm (supported)

Additional location

 Cytosol (supported)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
CAB026380 A-431 Nucleoplasm
Cytosol
HPA058267 HaCaT Nucleoplasm
Cytosol
HPA058267 RT4 Nucleoplasm
HPA058267 U-2 OS Nucleoplasm
Mitochondria Intensity

GENE INFORMATION

Gene name

 POU5F1 (HGNC Symbol)

Synonyms

 MGC22487, OCT3, Oct4, OTF3

Description

 POU class 5 homeobox 1 (HGNC Symbol)

Entrez gene summary

 This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

Chromosome

 6

Cytoband

 p21.33

Chromosome location (bp)

 31164337 - 31180731

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000204531 (version 83.38)

Entrez gene

 5460

UniProt

 Q01860 (UniProt - Evidence at protein level)

neXtProt

 NX_Q01860

Antibodypedia

 POU5F1 antibodies

PROTEIN BROWSER
POU5F1-001
POU5F1-002
POU5F1-004
POU5F1-005
POU5F1-006
POU5F1-007
POU5F1-201
POU5F1-202

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
POU5F1-001
ENSP00000259915
ENST00000259915
Q01860 [Direct mapping]
POU domain, class 5, transcription factor 1
D2IYK3 [Target identity:100%; Query identity:100%]
POU domain protein
Show all
Predicted intracellular proteins
Transcription factors
   Helix-turn-helix domains
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000122 [negative regulation of transcription from RNA polymerase II promoter]
GO:0000976 [transcription regulatory region sequence-specific DNA binding]
GO:0000981 [RNA polymerase II transcription factor activity, sequence-specific DNA binding]
GO:0001227 [transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding]
GO:0001714 [endodermal cell fate specification]
GO:0001824 [blastocyst development]
GO:0003130 [BMP signaling pathway involved in heart induction]
GO:0003677 [DNA binding]
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005667 [transcription factor complex]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0006366 [transcription from RNA polymerase II promoter]
GO:0008134 [transcription factor binding]
GO:0009611 [response to wounding]
GO:0009653 [anatomical structure morphogenesis]
GO:0009786 [regulation of asymmetric cell division]
GO:0010468 [regulation of gene expression]
GO:0031625 [ubiquitin protein ligase binding]
GO:0035019 [somatic stem cell population maintenance]
GO:0035198 [miRNA binding]
GO:0035413 [positive regulation of catenin import into nucleus]
GO:0042789 [mRNA transcription from RNA polymerase II promoter]
GO:0043565 [sequence-specific DNA binding]
GO:0044212 [transcription regulatory region DNA binding]
GO:0044822 [poly(A) RNA binding]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0060391 [positive regulation of SMAD protein import into nucleus]
GO:0060795 [cell fate commitment involved in formation of primary germ layer]
GO:0060913 [cardiac cell fate determination]
GO:0060965 [negative regulation of gene silencing by miRNA]
GO:0090081 [regulation of heart induction by regulation of canonical Wnt signaling pathway]
GO:0090308 [regulation of methylation-dependent chromatin silencing]
Show all
 360 aa
38.6 kDa 		No 0
POU5F1-002
ENSP00000425083
ENST00000471529
F2Z381 [Direct mapping]
POU class 5 homeobox 1 transcript variant 6; POU class 5 homeobox 1 transcript variant OCT4B1; POU domain, class 5, transcription factor 1
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003677 [DNA binding]
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005634 [nucleus]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0043565 [sequence-specific DNA binding]
Show all
 164 aa
18.3 kDa 		No 0
POU5F1-004
ENSP00000389359
ENST00000441888
F2Z381 [Direct mapping]
POU class 5 homeobox 1 transcript variant 6; POU class 5 homeobox 1 transcript variant OCT4B1; POU domain, class 5, transcription factor 1
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003677 [DNA binding]
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005634 [nucleus]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0043565 [sequence-specific DNA binding]
Show all
 164 aa
18.3 kDa 		No 0
POU5F1-005
ENSP00000475512
ENST00000513407
F2Z381 [Direct mapping]
POU class 5 homeobox 1 transcript variant 6; POU class 5 homeobox 1 transcript variant OCT4B1; POU domain, class 5, transcription factor 1
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003677 [DNA binding]
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005634 [nucleus]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0043565 [sequence-specific DNA binding]
Show all
 164 aa
18.3 kDa 		No 0
POU5F1-006
ENSP00000425479
ENST00000512818
F2Z381 [Direct mapping]
POU class 5 homeobox 1 transcript variant 6; POU class 5 homeobox 1 transcript variant OCT4B1; POU domain, class 5, transcription factor 1
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003677 [DNA binding]
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005634 [nucleus]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0043565 [sequence-specific DNA binding]
Show all
 164 aa
18.3 kDa 		No 0
POU5F1-007
ENSP00000475880
ENST00000606567
U3KQH6 [Direct mapping]
POU domain protein
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003677 [DNA binding]
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005634 [nucleus]
GO:0006351 [transcription, DNA-templated]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0043565 [sequence-specific DNA binding]
Show all
 190 aa
21.2 kDa 		No 0
POU5F1-201
ENSP00000481679
ENST00000619340
A0A087WYB7 [Direct mapping]
POU domain, class 5, transcription factor 1
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003677 [DNA binding]
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0006355 [regulation of transcription, DNA-templated]
Show all
 186 aa
19.1 kDa 		No 0
POU5F1-202
ENSP00000484778
ENST00000620031
U3KQH6 [Direct mapping]
POU domain protein
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003677 [DNA binding]
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005634 [nucleus]
GO:0006351 [transcription, DNA-templated]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0043565 [sequence-specific DNA binding]
Show all
 190 aa
21.2 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.