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Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Antibody dilution
1:100
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA018295
Antibody HPA020339
Antibody HPA021146
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA021146 across 75 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA021146 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA020339 across 74 cell types. Spearman correlation with HPA018295 across 75 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 53 cell types
RNA: detected in 37 tissues Protein: detected in 61 cell types
RNA: detected in 37 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:250
1:800
1:400
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018295
Antibody HPA020339
Antibody HPA021146
Formal validation: Genetic
Validated
Downregulation visible in one of two siRNA lanes
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: siRNA 1 Lane 3: siRNA 2 Lane 4: Scrambled
