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Immunofluorescent staining of human cell line CACO-2 shows localization to nucleus.
Antibody dilution
1:70
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA042763
Antibody HPA043615
Antibody HPA064578
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong positivity in cells in glomeruli and moderate staining in cells in tubules.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
Expression
RNA: detected in 28 tissues Protein: detected in 46 cell types
RNA: detected in 28 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:25
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042763
Antibody HPA043615
Antibody HPA064578
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY422124)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY422124)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
78.7, 78.1
78.7, 78.1, 19.6
78.7, 78.1
Antibody dilution
1:250
1:125
1:230
PROTEIN ARRAY
Antibody HPA042763
Antibody HPA043615
Antibody HPA064578
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
