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Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
Antibody dilution
1:100
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA018934
Antibody HPA021653
Antibody HPA029570
Standard validation
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human testis shows cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human colon shows distinct membranous positivity in glandular cells.
Expression
RNA: detected in 32 tissues Protein: detected in 66 cell types
RNA: detected in 32 tissues Protein: detected in 28 cell types
RNA: detected in 32 tissues Protein: detected in 71 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:250
1:75
1:60
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018934
Antibody HPA021653
Antibody HPA029570
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
66.5
66.5, 33.2
66.5
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA018934
Antibody HPA021653
Antibody HPA029570
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
