HN1L - Antibodies - The Human Protein Atlas

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HN1L

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA041888

Antibody HPA041908

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA041888

HPA041908

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA041888

Antibody HPA041908

Immunocytochemistry

Immunohistochemistry

Western blot

Supported

Protein array

Supported

Approved

IMMUNOCYTOCHEMISTRY

Antibody HPA041888

Antibody HPA041908

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA041908.

Validated

Antibody staining overlaps with antibody HPA041888.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.

Antibody dilution

1:120

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA041888

Antibody HPA041908

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA041908 across 73 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA041888 across 73 cell types.

Standard validation

Approved

Figure description

Immunohistochemical staining of human stomach shows strong cytoplasmic positivity.

Immunohistochemical staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 53 cell types

RNA: detected in 37 tissues
Protein: detected in 59 cell types

Retrieval

HIER pH6

Antibody dilution

1:225

1:1200

Literature conformity

Partly consistent with gene/protein characterization data.

Consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA041888

Antibody HPA041908

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

23, 20.1, 18.9, 18.4, 17.6

23, 20.7, 20.1, 18.9, 18.4, 17.6, 12.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA041888

Antibody HPA041908

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA041888

Antibody HPA041908

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

FGSPVTATSRLAHPNKPKDHVFLCEGEEPKSDLKAARSIPAGAEPGEKGS
ARKAGPAKEQEPMPTVDSHEPRLGPRPRSHNKVLNPPGGKSSISF

GSRAMKPPGGESSNLFGSPEEATPSSRPNRMASNIFGPTEEPQNIPKRTN
PPGGKGSGIFDESTPVQTRQHLNPPGGKTSDI

Matching transcripts

HN1L-001 - ENSP00000248098 [100%]
HN1L-002 - ENSP00000457694 [100%]
HN1L-004 - ENSP00000372157 [100%]
HN1L-009 - ENSP00000372158 [100%]
HN1L-012 - ENSP00000457424 [94%]

HN1L-001 - ENSP00000248098 [100%]
HN1L-014 - ENSP00000454567 [100%]
HN1L-002 - ENSP00000457694 [98%]
HN1L-004 - ENSP00000372157 [98%]
HN1L-009 - ENSP00000372158 [95%]
HN1L-010 - ENSP00000454529 [95%]
HN1L-012 - ENSP00000457424 [95%]

Other gene match

ANTIGEN VIEW

HN1L-001	HN1L-002	HN1L-004	HN1L-009	HN1L-010	HN1L-012	HN1L-014

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.