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DIO2

ANTIBODY INFORMATION
 
 

Antibody HPA029544

  

Antibody HPA048002

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA029544   HPA048002  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA029544

  

Antibody HPA048002

  
Immunocytochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA029544

  

Antibody HPA048002

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:20   1:100  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA029544

  

Antibody HPA048002

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 34.8, 30.6   30.6  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA029544

  

Antibody HPA048002

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:4000  

ANTIGEN INFORMATION
 
 

Antibody HPA029544

  

Antibody HPA048002

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 131   74  

Antigen sequence

 
PPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAIPGDSSLSFEVK
KHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIV
QRQKIAYLGGKGPFSYNLQEVRHWLEKNFSK
  
EWRRMLTSEGLRCVWKSFLLDAYKQVKLGEDAPNSSVVHVSSTEGGDNSG
NGTQEKIAEGATCHLLDFASPERP
 

Matching transcripts

 DIO2-001 - ENSP00000451419 [100%]
DIO2-002 - ENSP00000405854 [100%]
DIO2-003 - ENSP00000450980 [100%]
  DIO2-001 - ENSP00000451419 [100%]
DIO2-002 - ENSP00000405854 [100%]
 

Other gene match

    
ANTIGEN VIEW
DIO2-001
DIO2-002
DIO2-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.