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Immunofluorescent staining of human cell line SH-SY5Y shows localization to mitochondria.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA062808
Antibody HPA068759
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 12 tissues Protein: detected in 29 cell types
Retrieval
HIER pH6
Antibody dilution
1:65
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA062808
Antibody HPA068759
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
217.9
217.9
Antibody dilution
1:80
1:1250
PROTEIN ARRAY
Antibody HPA062808
Antibody HPA068759
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:900
1:15050
ANTIGEN INFORMATION
Antibody HPA062808
Antibody HPA068759
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
43
Antigen sequence
TNVRWLHNDSVLLTVGGADTALMIWTREFVGTQESKLVDSEESDTDVEED
G
