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ARHGEF33

ANTIBODY INFORMATION
 
 

Antibody HPA034661

  

Antibody HPA041051

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA034661   HPA041051  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA034661

  

Antibody HPA041051

  
Immunocytochemistry

Approved    
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA034661

  

Antibody HPA041051

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line SiHa shows localization to vesicles.    

Antibody dilution

 1:67    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA034661

  

Antibody HPA041051

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.   Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in purkinje cells.  

Expression

 RNA: detected in 15 tissues
Protein: detected in 25 cell types		   RNA: detected in 15 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:3000  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA034661

  

Antibody HPA041051

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 97.5   97.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA034661

  

Antibody HPA041051

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA034661

  

Antibody HPA041051

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 72   64  

Antigen sequence

 
CLSADIQSKGHLPSGMWRQPKDGKEWGEEYVTKDHPDKLKEAGQGRHSSL
ENVLCETSLAAKRQTVALELLE
  
GSPFRSINIPEPVLPSEDFTNLLPSQAYEKAQESRSVHVGDSNVKGMMGP
GVNPTTPEAEENLK
 

Matching transcripts

 ARHGEF33-001 - ENSP00000387020 [100%]
ARHGEF33-201 - ENSP00000381780 [100%]
  ARHGEF33-001 - ENSP00000387020 [100%]
ARHGEF33-201 - ENSP00000381780 [100%]
 

Other gene match

    
ANTIGEN VIEW
ARHGEF33-001
ARHGEF33-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.