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ZNF407

ANTIBODY INFORMATION
 
 

Antibody HPA028255

  

Antibody HPA041673

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA028255   HPA041673  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA028255

  

Antibody HPA041673

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028255

  

Antibody HPA041673

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & plasma membrane.  

Antibody dilution

   1:52  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028255

  

Antibody HPA041673

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human bone marrow shows strong nuclear positivity in megakaryocytes.    

Expression

 RNA: detected in 36 tissues
Protein: detected in 72 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:10    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA028255

  

Antibody HPA041673

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 247.4, 202.4, 184.1   247.4, 202.4, 184.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA028255

  

Antibody HPA041673

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA028255

  

Antibody HPA041673

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 122   87  

Antigen sequence

 
PVLGNKEILMNSQHETEFILEEDGPASDSTVESSDVYETIISIDDKGQAM
YSFGRFDSSIIRIKNPEDGELIDQSEEGLIATGVRISELPLKDCAQGVKK
KKSEGSSIGESTRIRCDDCGFL
  
SRKLDTLVTSEGLLEKLESTKNTLQAAHGNSVTSRPRPERNILVLGNSFR
RRSSTFTLKGQAKKRFNLLGIKRGTSETQRMYMKHLR
 

Matching transcripts

 ZNF407-001 - ENSP00000299687 [100%]
ZNF407-002 - ENSP00000462348 [100%]
ZNF407-006 - ENSP00000463270 [100%]
ZNF407-201 - ENSP00000310359 [100%]
  ZNF407-001 - ENSP00000299687 [100%]
ZNF407-002 - ENSP00000462348 [100%]
ZNF407-006 - ENSP00000463270 [100%]
ZNF407-201 - ENSP00000310359 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZNF407-001
ZNF407-002
ZNF407-006
ZNF407-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.