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Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Antibody dilution
1:103
1:60
Literature conformity
The subcellular location is supported by literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA043271
Antibody HPA067321
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human soft tissue shows moderate cytoplasmic positivity in peripheral nerve.
Expression
RNA: detected in 10 tissues Protein: detected in 9 cell types
Retrieval
HIER pH6
Antibody dilution
1:600
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043271
Antibody HPA067321
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
16.7, 16.6, 10.8
16.7, 16.6, 16.4, 13.4, 12.2
Antibody dilution
1:250
1:200
PROTEIN ARRAY
Antibody HPA043271
Antibody HPA067321
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:6000
1:2400
ANTIGEN INFORMATION
Antibody HPA043271
Antibody HPA067321
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
24
Antigen sequence
CVSILCCKFCKQVLSSRGMKAVLLADTEIDLFSTDIPPTNAVDFTGRCYF
T