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Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Antibody dilution
1:94
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA048295
Antibody HPA071744
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 5 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA048295
Antibody HPA071744
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
40.6, 20.1
40.6, 33.9, 20.1
Antibody dilution
1:80
1:320
PROTEIN ARRAY
Antibody HPA048295
Antibody HPA071744
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
