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CT47A4

ANTIBODY INFORMATION
 
 

Antibody HPA042483

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042483  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 CT47A10 - ENSG00000224089 [100%]
CT47A6 - ENSG00000226023 [100%]
CT47A9 - ENSG00000226600 [100%]
CT47A12 - ENSG00000226685 [100%]
CT47A11 - ENSG00000226929 [100%]
CT47A7 - ENSG00000228517 [100%]
CT47A8 - ENSG00000230347 [100%]
CT47A3 - ENSG00000236126 [100%]
CT47A1 - ENSG00000236371 [100%]
CT47B1 - ENSG00000236446 [100%]
CT47A5 - ENSG00000237957 [100%]
CT47A2 - ENSG00000242362 [100%]
RP13-347D8.7 - ENSG00000277535 [82%]
RP1-321E8.5 - ENSG00000278646 [89%]
  

Released in version

 10  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA042483

  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042483

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic and nucleolar positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 31 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:750  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042483

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 35.1, 31.3, 30.1, 4.5  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA042483

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000  

ANTIGEN INFORMATION
 
 

Antibody HPA042483

  

Antigen

 Recombinant protein fragment  

Length (aa)

 28  

Antigen sequence

 
PEEVTKSQPEKWDEEAQDAAGEEEKEQE
 

Matching transcripts

 CT47A4-001 - ENSP00000389513 [100%]
 

Other gene match

 CT47A10 - ENSG00000224089 [100%]
CT47A6 - ENSG00000226023 [100%]
CT47A9 - ENSG00000226600 [100%]
CT47A12 - ENSG00000226685 [100%]
CT47A11 - ENSG00000226929 [100%]
CT47A7 - ENSG00000228517 [100%]
CT47A8 - ENSG00000230347 [100%]
CT47A3 - ENSG00000236126 [100%]
CT47A1 - ENSG00000236371 [100%]
CT47B1 - ENSG00000236446 [100%]
CT47A5 - ENSG00000237957 [100%]
CT47A2 - ENSG00000242362 [100%]
RP13-347D8.7 - ENSG00000277535 [82%]
RP1-321E8.5 - ENSG00000278646 [89%]
  
ANTIGEN VIEW
CT47A4-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.