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Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
Antibody dilution
1:47
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA051027
Antibody HPA061215
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human heart muscle shows distinct membranous and cytoplasmic positivity in myocytes. ( Intercalated disc distinctly stained ).
Expression
RNA: detected in 34 tissues Protein: detected in 33 cell types
Retrieval
HIER pH6
Antibody dilution
1:400
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051027
Antibody HPA061215
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
58, 49.9
58, 49.9
Antibody dilution
1:230
1:1100
PROTEIN ARRAY
Antibody HPA051027
Antibody HPA061215
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
