ARPIN - Antibodies - The Human Protein Atlas

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ARPIN

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA040513

Antibody HPA047173

Antibody HPA052278

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA040513

HPA047173

HPA052278

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

C15orf38-AP3S2 - ENSG00000250021 [100%]

C15orf38-AP3S2 - ENSG00000250021 [97%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA040513

Antibody HPA047173

Antibody HPA052278

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA040513

Antibody HPA047173

Antibody HPA052278

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 36 cell types

RNA: detected in 36 tissues
Protein: detected in 67 cell types

RNA: detected in 36 tissues
Protein: detected in 63 cell types

Retrieval

HIER pH6

Antibody dilution

1:35

1:100

1:400

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA040513

Antibody HPA047173

Antibody HPA052278

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

43.9, 24.9

43.9, 24.9, 20.4, 14.9, 14.1

Antibody dilution

1:250

1:350

1:480

PROTEIN ARRAY

Antibody HPA040513

Antibody HPA047173

Antibody HPA052278

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:2850

1:3900

ANTIGEN INFORMATION

Antibody HPA040513

Antibody HPA047173

Antibody HPA052278

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

MSRIYHDGALRNKAVQSVRLPGAWDPAAHQGGNGVLLEGELIDVSRHSIL
DTHGRKERYYVLYIRPSHIHRRKF

FDAKGNEIEPNFSATRKVNTGFLMSSYKVEAKGDTDRLTPEALKGLVNKP
ELLALTESLTPDHTVAFWMPESEME

EVMELELGAGVRLKTRGDGPFLDSLAKLEAGTVTKCNFTGDGKTGASWTD
NIMAQKCSKGAAAEIREQGDGAEDEEWDD

Matching transcripts

ARPIN-001 - ENSP00000350075 [100%]

ARPIN-001 - ENSP00000350075 [100%]
ARPIN-002 - ENSP00000453687 [100%]
ARPIN-003 - ENSP00000453006 [97%]

Other gene match

C15orf38-AP3S2 - ENSG00000250021 [100%]

C15orf38-AP3S2 - ENSG00000250021 [97%]

ANTIGEN VIEW

ARPIN-001	ARPIN-002	ARPIN-003

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.