AMY2A - Antibodies - The Human Protein Atlas

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AMY2A

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA045394

Antibody HPA045399

Antibody HPA046980

Antibody CAB045960

Provider

Atlas Antibodies
Sigma-Aldrich

Origene

Product name

HPA045394

HPA045399

HPA046980

TA500146

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

AMY1B - ENSG00000174876 [100%]
AMY1C - ENSG00000187733 [100%]
AMY1A - ENSG00000237763 [100%]
AMY2B - ENSG00000240038 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA045394

Antibody HPA045399

Antibody HPA046980

Antibody CAB045960

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA045394

Antibody HPA045399

Antibody HPA046980

Antibody CAB045960

Standard validation

Supported

Figure description

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine pancreas.

Expression

RNA: detected in 13 tissues
Protein: detected in 2 cell types

Retrieval

HIER pH6

Antibody dilution

1:600

1:400

1:300

1:2000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA045394

Antibody HPA045399

Antibody HPA046980

Antibody CAB045960

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

57.8, 57.7

57.8, 57.7, 25.6, 23.5

57.7, 23.5

Antibody dilution

1:250

1:80

1:500

PROTEIN ARRAY

Antibody HPA045394

Antibody HPA045399

Antibody HPA046980

Antibody CAB045960

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:600

ANTIGEN INFORMATION

Antibody HPA045394

Antibody HPA045399

Antibody HPA046980

Antibody CAB045960

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

SLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDP
FIAIHAESKL

KPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAKLGTVIRKWNGEKMSY
LKNWGEGWG

GNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGSGDIENYND
ATQVRDCRL

Matching transcripts

AMY2A-001 - ENSP00000397582 [100%]
AMY2A-201 - ENSP00000481450 [100%]

AMY2A-001 - ENSP00000397582 [100%]
AMY2A-201 - ENSP00000481450 [100%]
AMY2A-002 - ENSP00000390832 [93%]

Other gene match

AMY1B - ENSG00000174876 [100%]
AMY1C - ENSG00000187733 [100%]
AMY1A - ENSG00000237763 [100%]
AMY2B - ENSG00000240038 [100%]

ANTIGEN VIEW

AMY2A-001	AMY2A-002	AMY2A-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.