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ZNF512

ANTIBODY INFORMATION
 
 

Antibody HPA031430

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA031430  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 6  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA031430

  
Immunocytochemistry

Approved  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA031430

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:60  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  
 

Validation: Genetic

 Supported

Significant downregulation 10-25% by one/two siRNA:s		  

 

Relative fluorescence intensity (RFI)

  

Figure description

 Nuclear region of segmented cells in 10x-images  

Antibody dilution

 1:60  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031430

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human ovary shows nuclear positivity in ovarian stroma cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 61 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:135  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA031430

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

 

Figure description

  

Target mass (kDa)

 64.7, 64.6, 56.4  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA031430

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA031430

  

Antigen

 Recombinant protein fragment  

Length (aa)

 91  

Antigen sequence

 
PKTQPNPKSQARRIRKEPPVYAAGSLEEQWYLEIVDKGSVSCPTCQAVGR
KTIEGLKKHMENCKQEMFTCHHCGKQLRSLAGMKYHVMANH
 

Matching transcripts

 ZNF512-001 - ENSP00000347648 [100%]
ZNF512-002 - ENSP00000369040 [100%]
ZNF512-201 - ENSP00000395660 [100%]
ZNF512-203 - ENSP00000451572 [100%]
 

Other gene match

  
ANTIGEN VIEW
ZNF512-001
ZNF512-002
ZNF512-201
ZNF512-203
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.