ACY1 - Antibodies - The Human Protein Atlas

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ACY1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA036174

Antibody HPA036175

Antibody CAB003695

Provider

Atlas Antibodies
Sigma-Aldrich

Abnova

Product name

HPA036174

HPA036175

H00000095-M01

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

ABHD14A-ACY1 - ENSG00000114786 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA036174

Antibody HPA036175

Antibody CAB003695

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA036174

Antibody HPA036175

Antibody CAB003695

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in renal tubules.

Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in subsets of renal tubules.

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 6 cell types

RNA: detected in 37 tissues
Protein: detected in 18 cell types

RNA: detected in 37 tissues
Protein: detected in 2 cell types

Retrieval

HIER pH6

Antibody dilution

1:2000

1:350

1:500

Literature conformity

Consistent with extensive gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036174

Antibody HPA036175

Antibody CAB003695

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

56.4, 45.9, 42.2, 38.6, 25.8

56.4, 45.9, 42.2, 37.6

45.9, 42.2, 38.6, 37.6, 25.8

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA036174

Antibody HPA036175

Antibody CAB003695

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA036174

Antibody HPA036175

Antibody CAB003695

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

QGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERSPWWVRVTSTG
RPGHASRFMEDTAAEKLHKVVNSILAFRE

SVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQA
AGEGVTLEFAQKWMHPQVTPTDDSNPWWAAFSRVCK

Matching transcripts

ACY1-001 - ENSP00000384296 [100%]
ACY1-007 - ENSP00000417056 [100%]
ACY1-006 - ENSP00000419262 [85%]
ACY1-011 - ENSP00000419830 [85%]

ACY1-001 - ENSP00000384296 [100%]
ACY1-007 - ENSP00000417056 [100%]
ACY1-008 - ENSP00000417618 [100%]

Other gene match

ABHD14A-ACY1 - ENSG00000114786 [100%]

ANTIGEN VIEW

ACY1-001	ACY1-006	ACY1-007	ACY1-008	ACY1-011

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.