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Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Expression
RNA: detected in 0 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA012298
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
28.9, 19.4
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA012298
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
