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SPATS1

ANTIBODY INFORMATION
 
 

Antibody HPA030897

  

Antibody HPA046230

  

Antibody HPA049722

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA030897   HPA046230   HPA049722  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 16   11   11  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA030897

  

Antibody HPA046230

  

Antibody HPA049722

  
Immunocytochemistry

Approved      
Immunohistochemistry

  Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030897

  

Antibody HPA046230

  

Antibody HPA049722

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.      

Antibody dilution

 1:6      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030897

  

Antibody HPA046230

  

Antibody HPA049722

  

Standard validation

   Approved   Approved  

     

Figure description

   Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous staining in a subset of renal tubules   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.  

Expression

   RNA: detected in 3 tissues
Protein: detected in 27 cell types		   RNA: detected in 3 tissues
Protein: detected in 33 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:450   1:250  

Literature conformity

   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

   Not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030897

  

Antibody HPA046230

  

Antibody HPA049722

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 33.7, 12.5   33.7   33.7, 12.5  

Antibody dilution

 1:250   1:250   1:1010  

PROTEIN ARRAY
 
 

Antibody HPA030897

  

Antibody HPA046230

  

Antibody HPA049722

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000   1:8050  

ANTIGEN INFORMATION
 
 

Antibody HPA030897

  

Antibody HPA046230

  

Antibody HPA049722

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 74   64   74  

Antigen sequence

 
SPSMLTGNSPRGCRLPSISSTTCGRQLEKVPEKRDSGMTEVERTYSANCS
DFLESKGCFANTTPSGKSVSSSSS
  
VPYEKKFDTFIPLEPLPQIPNLPFWVKEKANSLKNEIQEVEELDNWQPAV
PLMHMLHLSGALDF
  
SPSMLTGNSPRGCRLPSISSTTCGRQLEKVPEKRDSGMTEVERTYSANCS
DFLESKGCFANTTPSGKSVSSSSS
 

Matching transcripts

 SPATS1-001 - ENSP00000424400 [100%]
SPATS1-003 - ENSP00000423654 [100%]
SPATS1-201 - ENSP00000437552 [100%]
  SPATS1-001 - ENSP00000424400 [100%]
SPATS1-201 - ENSP00000437552 [100%]
  SPATS1-001 - ENSP00000424400 [100%]
SPATS1-003 - ENSP00000423654 [100%]
SPATS1-201 - ENSP00000437552 [100%]
 

Other gene match

      
ANTIGEN VIEW
SPATS1-001
SPATS1-003
SPATS1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.