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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Antibody dilution
1:125
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody CAB019384
Standard validation
Approved
Figure description
Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.
Expression
RNA: detected in 20 tissues Protein: detected in 71 cell types
Retrieval
HIER pH6
Antibody dilution
1:2200
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody CAB019384
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
