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RP11-849H4.2

ANTIBODY INFORMATION
 
 

Antibody HPA051121

  

Antibody HPA058841

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA051121   HPA058841  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA051121

  

Antibody HPA058841

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051121

  

Antibody HPA058841

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line HaCaT shows localization to nucleoli.   Immunofluorescent staining of human cell line HaCaT shows localization to nucleoli.  

Antibody dilution

 1:102   1:4  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051121

  

Antibody HPA058841

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human stomach, lower shows moderate cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 34 tissues
Protein: detected in 41 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:1300    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA051121

  

Antibody HPA058841

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 15.8   15.8, 14.8, 9.9, 9  

Antibody dilution

 1:510   1:80  

PROTEIN ARRAY
 
 

Antibody HPA051121

  

Antibody HPA058841

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:15000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA051121

  

Antibody HPA058841

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 71   71  

Antigen sequence

 
QIDVGHSSWPLDRPFITLLPATTLMSLTDSKQGKNRSGVRMFKDGKEGKS
RKDGGGLYEKQRCSTKEDCEC
  
MAPVKISHVVSFSSQDPKYPVENLLNPDSPRRPWLGCPQDKSGQLKVELQ
LERAVPTGYIDVGNCGCAFLQ
 

Matching transcripts

 RP11-849H4.2-001 - ENSP00000433080 [100%]
  RP11-849H4.2-001 - ENSP00000433080 [100%]
RP11-849H4.2-002 - ENSP00000435620 [100%]
RP11-849H4.2-006 - ENSP00000435060 [100%]
RP11-849H4.2-008 - ENSP00000436412 [100%]
 

Other gene match

    
ANTIGEN VIEW
RP11-849H4.2-001
RP11-849H4.2-002
RP11-849H4.2-006
RP11-849H4.2-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.