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Immunohistochemical staining of human pancreas shows cytoplasmic positivity in islets of Langerhans.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
Expression
RNA: detected in 20 tissues Protein: detected in 2 cell types
RNA: detected in 20 tissues Protein: detected in 2 cell types
RNA: detected in 20 tissues Protein: detected in 39 cell types
RNA: detected in 20 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
None
HIER pH6
HIER pH6
Antibody dilution
1:750
1:1000
1:3000
1:600
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004932
Antibody CAB000048
Antibody CAB012098
Antibody CAB053843
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400078)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
12, 11
12, 11, 10.6
12, 11, 10.6
12, 11, 10.6
Antibody dilution
1:250
1:500
1:500
1:500
PROTEIN ARRAY
Antibody HPA004932
Antibody CAB000048
Antibody CAB012098
Antibody CAB053843
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
