Fields »
ABOUT
HELP
BLOG
CHMP1B

ANTIBODY INFORMATION
 
 

Antibody HPA061997

  

Antibody HPA062896

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA061997   HPA062896  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA061997

  

Antibody HPA062896

  
Immunocytochemistry

Supported    
Immunohistochemistry


Validated
   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA061997

  

Antibody HPA062896

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & midbody.    

Antibody dilution

 1:122    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA061997

  

Antibody HPA062896

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells and islets of Langerhans.   Immunohistochemical staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 64 cell types		   RNA: detected in 37 tissues
Protein: detected in 55 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:2000   1:800  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA061997

  

Antibody HPA062896

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 22.1   22.1  

Antibody dilution

 1:410   1:460  

PROTEIN ARRAY
 
 

Antibody HPA061997

  

Antibody HPA062896

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:4900   1:5500  

ANTIGEN INFORMATION
 
 

Antibody HPA061997

  

Antibody HPA062896

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 54   60  

Antigen sequence

 
QNQVDMLLQEMADEAGLDLNMELPQGQTGSVGTSVASAEQDELSQRLARL
RDQV
  
TAVTMGKVTKSMAGVVKSMDATLKTMNLEKISALMDKFEHQFETLDVQTQ
QMEDTMSSTT
 

Matching transcripts

 CHMP1B-001 - ENSP00000432279 [100%]
  CHMP1B-001 - ENSP00000432279 [100%]
 

Other gene match

    
ANTIGEN VIEW
CHMP1B-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.