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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:44
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA011188
Antibody HPA053611
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Expression
RNA: detected in 3 tissues Protein: detected in 72 cell types
RNA: detected in 3 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:20
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011188
Antibody HPA053611
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil