HP - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA047750

Antibody CAB003787

Provider

Atlas Antibodies
Sigma-Aldrich

HUPO-liver

Product name

HPA047750

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

HPR - ENSG00000261701 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA047750

Antibody CAB003787

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA047750

Antibody CAB003787

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & vesicles.

Antibody dilution

1:26

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA047750

Antibody CAB003787

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Expression

RNA: detected in 29 tissues
Protein: detected in 35 cell types

RNA: detected in 29 tissues
Protein: detected in 9 cell types

Retrieval

HIER pH6

Antibody dilution

1:45

1:100

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA047750

Antibody CAB003787

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

49.1, 45.2, 44.9, 39, 38.5, 31.5, 31.4

49.1, 45.2, 44.9, 40.7, 38.5, 31.4, 24.8, 16.5, 10.2, 9.9, 9.7

Antibody dilution

1:130

1:500

PROTEIN ARRAY

Antibody HPA047750

Antibody CAB003787

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA047750

Antibody CAB003787

Antigen

Recombinant protein fragment

Native protein

Length (aa)

Antigen sequence

GKPKNPANPVQRILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLT
TAKNLFLNHSENATAKDIAPTLTLYVG

Matching transcripts

HP-001 - ENSP00000457629 [100%]
HP-002 - ENSP00000348170 [100%]
HP-003 - ENSP00000454966 [100%]
HP-012 - ENSP00000350406 [100%]
HP-016 - ENSP00000464070 [100%]
HP-202 - ENSP00000381199 [100%]
HP-201 - ENSP00000228226 [91%]
HP-203 - ENSP00000478279 [91%]

Other gene match

HPR - ENSG00000261701 [100%]

ANTIGEN VIEW

HP-001	HP-002	HP-003	HP-012	HP-016	HP-201	HP-202	HP-203

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.