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ANTIBODY INFORMATION
 
 

Antibody HPA002270

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002270  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 3  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA002270

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002270

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human duodenum shows strong membranous and moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 11 tissues
Protein: detected in 25 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002270

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 312, 209.8  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA002270

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2000  

ANTIGEN INFORMATION
 
 

Antibody HPA002270

  

Antigen

 Recombinant protein fragment  

Length (aa)

 126  

Antigen sequence

 
VYLLCEFSVTQNRLEVNISQSTYKDPNNLAFNEIKILGTEEPSNVTVKHN
GVPSQTSPTVTYDSNLKVAIITDIDLLLGEAYTVEWSIKIRDEEKIDCYP
DENGASAENCTARGCIWEASNSSGVP
 

Matching transcripts

 MGAM-001 - ENSP00000447378 [100%]
MGAM-007 - ENSP00000417515 [100%]
MGAM-201 - ENSP00000482292 [100%]
 

Other gene match

  
ANTIGEN VIEW
MGAM-001
MGAM-007
MGAM-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.