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SYNJ2BP-COX16

ANTIBODY INFORMATION
 
 

Antibody HPA000866

  

Antibody HPA062659

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000866   HPA062659  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 SYNJ2BP - ENSG00000213463 [100%]
   COX16 - ENSG00000133983 [100%]
  

Released in version

 1   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA000866

  

Antibody HPA062659

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000866

  

Antibody HPA062659

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.   Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.  

Antibody dilution

 1:83   1:76  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000866

  

Antibody HPA062659

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human smooth muscle shows moderate cytoplasmic and membranous positivity in smooth muscle cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 36 cell types		   RNA: detected in 36 tissues
Protein: detected in 15 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:200  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Not consistent with RNA expression data.  
 

Standard validation

 Supported    

   

Figure description

 Strong labelling of synaptic fields in the hippocampus, cortex and periaquaductal grey.    

WESTERN BLOT
 
 

Antibody HPA000866

  

Antibody HPA062659

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 20.8, 20.5, 17.3, 15.9   20.8, 20.5, 17.3, 12.3, 6.5  

Antibody dilution

 1:500   1:250  

PROTEIN ARRAY
 
 

Antibody HPA000866

  

Antibody HPA062659

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1000   1:3050  

ANTIGEN INFORMATION
 
 

Antibody HPA000866

  

Antibody HPA062659

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 112   36  

Antigen sequence

 
NGRVDYLVTEEEINLTRGPSGLGFNIVGGTDQQYVSNDSGIYVSRIKENG
AAALDGRLQEGDKILSVNGQDLKNLLHQDAVDLFRNAGYAVSLRVQHRLQ
VQNGPIGHRGEG
  
IKDSKFDDWKNIRGPRPWEDPDLLQGRNPESLKTKT
 

Matching transcripts

 SYNJ2BP-COX16-003 - ENSP00000478757 [100%]
SYNJ2BP-COX16-002 - ENSP00000482133 [91%]
SYNJ2BP-COX16-005 - ENSP00000484161 [88%]
  SYNJ2BP-COX16-002 - ENSP00000482133 [100%]
SYNJ2BP-COX16-001 - ENSP00000480746 [89%]
SYNJ2BP-COX16-003 - ENSP00000478757 [89%]
SYNJ2BP-COX16-005 - ENSP00000484161 [89%]
 

Other gene match

 SYNJ2BP - ENSG00000213463 [100%]
   COX16 - ENSG00000133983 [100%]
  
ANTIGEN VIEW
SYNJ2BP-COX16-001
SYNJ2BP-COX16-002
SYNJ2BP-COX16-003
SYNJ2BP-COX16-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.