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ANTIBODY INFORMATION
 
 

Antibody HPA051257

  

Antibody CAB026357

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA051257   3456.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 12   5  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA051257

  

Antibody CAB026357

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051257

  

Antibody CAB026357

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 24 tissues
Protein: detected in 17 cell types		   RNA: detected in 24 tissues
Protein: detected in 10 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:3500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051257

  

Antibody CAB026357

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 12.9   12.9, 8.8  

Antibody dilution

 1:370   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051257

  

Antibody CAB026357

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2950    

ANTIGEN INFORMATION
 
 

Antibody HPA051257

  

Antibody CAB026357

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 64    

Antigen sequence

 
LKGNKHPINSEWQTDNCETCTCYETEISCCTLVSTPVGYDKDNCQRIFKK
EDCKYIVVEKKDPK
   

Matching transcripts

 MSMB-001 - ENSP00000463092 [100%]
   

Other gene match

    
ANTIGEN VIEW
MSMB-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.